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Serology - Blood Type Analysis Goal: Determine blood type of an unknown by adding anti-serum. Objective: · Acquaint the students with the ABO system of blood group.· Identify different blood type by using appropriate antigen. · Match unknown blood type to appropriate blood group. · Identify blood type from both dry and wet blood. Equipment:
Procedure: 1. Instructor must go over “Safety Biohazard” 2. Instructor must require the students to wear safety equipments before they touch any instruments, especially blood samples. 3. Instructor will hand out victim and suspects’ blood in small vials to each group of students. 4. Students will place 2 drops of the 1st sample of blood on each (two) microscope slides. 5. Add a drop of Anti-A serum to the 1st slide and stir it with a mixing stick. Immediately, dispose the stick in proper container. 6. Add a drop of Anti-B serum to the 2nd slide and stir it with a new mixing stick. Immediately, dispose the stick. 7. Observe the clumping and determine blood type according to the given chart in Background/Theory page. 8. Repeat the procedure for other samples. 9. Students will have to identify the blood type of evidence that has been collected from the crime scene. 10. Deduce the unknown ABO blood types 11. Make sure to disinfect the work area every experiment. Analysis and Conclusion: · You just learned the ABO blood type testing method. In the real world, would this test be perfectly valid at the crime scene?· Can you explain the ABO blood type antigen/antibody interaction? · Based on antigen/antibody reaction, How to distinguish blood samples from human and animal? Activity Length: · 1 class period for Biohazard Safety Rule· 1 class period for theory and background · 2 lab periods for experimentation and data collection · 1 class period for analyzing and group discussion. · Research assignment time – presentation and conclusion
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